![]() ![]() The BCO administration reduced cortisol level significantly (P<0.05) in heat-stressed broilers. This study found that application of heat stress to broiler chickens increased cortisol levels and induced histopathological changes in the liver. The data were analyzed using one way analysis of variance (ANOVA). Liver histopathology was stained with hematoxylin eosin and observed with electron microscope. Cortisol was measured using the cortisol enzyme-linked immunosorbent assay (ELISA) kit. Heat stress was given for 5 hours with temperature range of 34-35° C for 7 days. A total of 15 broiler chickens were used in this study and divided into 5 groups, K- (without treatment), K+ (given heat stress), P1 (given heat stress and 0.56mL BCO/400kg body weight, P2 (given heat stress and 1.11 mL BCO/400 g body weight), and P3 (given heat stress and 2.22 mL BCO/400 g body weight). The aim of this study was to observe cortisol levels and liver histopathology of broiler chicken that were treated with black cumin oil (BCO) under heat stress.
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